LOCUS       40924_23799       13119 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB733 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13119)
  AUTHORS   Tanaka Y, Nakamura S, Kawamukai M, Koizumi N, Nakagawa T.
  TITLE     Development of a series of gateway binary vectors possessing a 
            tunicamycin resistance gene as a marker for the transformation of 
            Arabidopsis thaliana
  JOURNAL   Biosci. Biotechnol. Biochem. 75 (4), 804-807 (2011)
  PUBMED    21512216
REFERENCE   2  (bases 1 to 13119)
  AUTHORS   Tanaka Y, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 13119)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13119)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem."; date: "2011"; volume: "75"; issue: "4"; 
            pages: "804-807"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..13119
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    323..447
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        484..514
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             568..1224
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             1569..1871
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(1915..2039)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2050..3858
                     /codon_start=1
                     /label=GUS
                     /note="beta-glucuronidase"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     terminator      3883..4135
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4169..4185)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4193..4209
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4217..4247)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4262..4283)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(4319..4571)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4609..5904)
                     /codon_start=1
                     /gene="gpt"
                     /product="UDP-N-acetylglucosamine: dolichol phosphate 
                     N-acetylglucosamine-1-P transferase"
                     /label=gpt
                     /protein_id="BAJ61248.1"
                     /translation="MAARKRASSISIPNKPDPSEPNSAPSEQKMTRKTVSASGEEFRLA
                     PPKLGVIFVISTLLCSLYLYLLCFHYKVDNELKRSILINAGLSLVGFFVTLKMIPVTAR
                     YVLRRNMFGFDINKRGTPQGDIKVPESLGIVVGIVFLIVAIIFQYFNFTEDSNWLVEYN
                     AALASICFMILLGFVDDVLDVPWRVKLVLPSFATLPLLMAYAGHTTIVIPKPLVAYIGL
                     EVLNLGRIYKLYMGLLAVFCTNSINIHAGLNGLEIGQTVVIAAAILIHNVMQIGASVDP
                     EYHQAHAFSIFLTQPLMATSLAMLAYNWYPSSVFVGDTYTVFAGMTMAVVGILGHFSET
                     LLIFFLPQVLNLLLSLPQLAGIVKCPRHRLPRYDPATGLLTGTKDGTLVNVYLRLFGPK
                     SEKSLCIHLLVFQALACAFCFILRHFLAGWYK"
     gene            complement(4609..5904)
                     /gene="gpt"
                     /label=gpt
     promoter        complement(5956..6135)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(6641..6665)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7186..7974
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8223..8811
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8997..9137)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9481..9675)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9744..10814)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11246..11872)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgggt ctagagttat caacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
      361 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
      421 taaaacacaa catatccagt cactatggcg gcccctcgag gggccgcatt aggcacccca
      481 ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg
      541 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
      601 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
      661 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
      721 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
      781 gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
      841 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
      901 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
      961 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg
     1021 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
     1081 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
     1141 catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
     1201 tgcgatgagt ggcagggcgg ggcgtaaaga tctggatccg gcttactaaa agccagataa
     1261 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc
     1321 cgaagtatgt caaaaagagg tatgctatga agcagcgtat tacagtgaca gttgacagcg
     1381 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac
     1441 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg
     1501 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacagggg
     1561 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg
     1621 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca
     1681 gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg
     1741 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg
     1801 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
     1861 tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt
     1921 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
     1981 aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtt
     2041 gataacagca tgttacgtcc tgtagaaacc ccaacccgtg aaatcaaaaa actcgacggc
     2101 ctgtgggcat tcagtctgga tcgcgaaaac tgtggaattg atcagcgttg gtgggaaagc
     2161 gcgttacaag aaagccgggc aattgctgtg ccaggcagtt ttaacgatca gttcgccgat
     2221 gcagatattc gtaattatgc gggcaacgtc tggtatcagc gcgaagtctt tataccgaaa
     2281 ggttgggcag gccagcgtat cgtgctgcgt ttcgatgcgg tcactcatta cggcaaagtg
     2341 tgggtcaata atcaggaagt gatggagcat cagggcggct atacgccatt tgaagccgat
     2401 gtcacgccgt atgttattgc cgggaaaagt gtacgtatca ccgtttgtgt gaacaacgaa
     2461 ctgaactggc agactatccc gccgggaatg gtgattaccg acgaaaacgg caagaaaaag
     2521 cagtcttact tccatgattt ctttaactat gccggaatcc atcgcagcgt aatgctctac
     2581 accacgccga acacctgggt ggacgatatc accgtggtga cgcatgtcgc gcaagactgt
     2641 aaccacgcgt ctgttgactg gcaggtggtg gccaatggtg atgtcagcgt tgaactgcgt
     2701 gatgcggatc aacaggtggt tgcaactgga caaggcacta gcgggacttt gcaagtggtg
     2761 aatccgcacc tctggcaacc gggtgaaggt tatctctatg aactgtgcgt cacagccaaa
     2821 agccagacag agtgtgatat ctacccgctt cgcgtcggca tccggtcagt ggcagtgaag
     2881 ggcgaacagt tcctgattaa ccacaaaccg ttctacttta ctggctttgg tcgtcatgaa
     2941 gatgcggact tgcgtggcaa aggattcgat aacgtgctga tggtgcacga ccacgcatta
     3001 atggactgga ttggggccaa ctcctaccgt acctcgcatt acccttacgc tgaagagatg
     3061 ctcgactggg cagatgaaca tggcatcgtg gtgattgatg aaactgctgc tgtcggcttt
     3121 aacctctctt taggcattgg tttcgaagcg ggcaacaagc cgaaagaact gtacagcgaa
     3181 gaggcagtca acggggaaac tcagcaagcg cacttacagg cgattaaaga gctgatagcg
     3241 cgtgacaaaa accacccaag cgtggtgatg tggagtattg ccaacgaacc ggatacccgt
     3301 ccgcaaggtg cacgggaata tttcgcgcca ctggcggaag caacgcgtaa actcgacccg
     3361 acgcgtccga tcacctgcgt caatgtaatg ttctgcgacg ctcacaccga taccatcagc
     3421 gatctctttg atgtgctgtg cctgaaccgt tattacggat ggtatgtcca aagcggcgat
     3481 ttggaaacgg cagagaaggt actggaaaaa gaacttctgg cctggcagga gaaactgcat
     3541 cagccgatta tcatcaccga atacggcgtg gatacgttag ccgggctgca ctcaatgtac
     3601 accgacatgt ggagtgaaga gtatcagtgt gcatggctgg atatgtatca ccgcgtcttt
     3661 gatcgcgtca gcgccgtcgt cggtgaacag gtatggaatt tcgccgattt tgcgacctcg
     3721 caaggcatat tgcgcgttgg cggtaacaag aaagggatct tcactcgcga ccgcaaaccg
     3781 aagtcggcgg cttttctgct gcaaaaacgc tggactggca tgaacttcgg tgaaaaaccg
     3841 cagcagggag gcaaacaatg agcttagagc tcgaatttcc ccgatcgttc aaacatttgg
     3901 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt
     3961 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
     4021 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata
     4081 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa
     4141 ttggttccgg aaccaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     4201 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     4261 aatgagtgag ctaactcaca ttaggctgaa ttaggcgcgc ctatttctat taattcccga
     4321 tctagtaaca tagatgacac cgcgcgcgat aatttatcct agtttgcgcg ctatattttg
     4381 ttttctatcg cgtattaaat gtataattgc gggactctaa tcataaaaac ccatctcata
     4441 aataacgtca tgcattacat gttaattatt acatgcttaa cgtaattcaa cagaaattat
     4501 atgataatca tcgcaagacc ggcaacagga ttcaatctta agaaacttta ttgccaaatg
     4561 tttgaacgat cggggaaatt cgcctcgagt gacttctctg attgcagact atttatacca
     4621 accagcaaga aagtgtcgaa gtatgaagca gaaggcgcag gctagagcct ggaaaacgag
     4681 aagatggatg caaagagact tttctgattt aggaccaaac aacctcaagt aaacattgac
     4741 gagtgtccca tcttttgtgc cggtcagtaa tccggtagca ggatcatacc ttgggagacg
     4801 atggcgcgga catttcacaa tcccagcaag ctgaggaagc gataatagaa ggttcagaac
     4861 ttgaggaaga aagaagatga ggagggtttc actgaagtga cccagaatac caacaactgc
     4921 catagtcatt ccggcgaaga ctgtgtaagt gtctccaaca aaaactgaag aaggatacca
     4981 attgtaagca agcattgcca aggatgtggc catcagcggt tgagtaagaa atatcgagaa
     5041 ggcatgagct tggtgatact cgggatcaac agatgctccg atttgcataa cgttgtgtat
     5101 caggatagca gcggcaataa caactgtttg accgatttca aggccattca gaccagcgtg
     5161 gatgttaata gagtttgtac aaaagacagc aagtagcccc atatataact tatatatcct
     5221 tcctagatta agcacttcca agccaatata agcaacaaga ggttttggta taacaatagt
     5281 tgtatgacca gcataagcca tcaaaagtgg aagggtagca aaagatggca ggacaagttt
     5341 tactctccaa ggcacatcaa ggacatcatc tacaaatcca agcaaaatca tgaagcaaat
     5401 agatgctagt gctgcattat actccacaag ccaattagaa tcttcagtga agttaaaata
     5461 ctgaaatatt atcgccacga tcaaaaagac aataccaaca acaataccca atgactctgg
     5521 cactttaata tctccttgag gcgtgcctct tttgttgata tcaaaaccaa acatgttacg
     5581 tctcagaacg tatctggcag taacaggaat cattttgaga gtgacgaaaa acccaaccaa
     5641 gctaagcccc gcgttaatta gaatcgatcg cttgagttca ttatcaacct tgtagtgaaa
     5701 acagagaagg tatagataga gcgaacagag aagagtagag atcacgaaga tcactcctaa
     5761 tttcggcggc gcgagacgaa attcctcacc ggaagcagaa accgtctttc gggtcatctt
     5821 ctgctccgat ggcgctgagt tgggttccga cgggtccggt ttgttgggaa ttgagatcga
     5881 tgaagctctc ttacgagctg ccatatctta ttgccccccg ggatcagatc tggattgaga
     5941 gtgaatatga gactctaatt ggataccgag gggaatttat ggaacgtcag tggagcattt
     6001 ttgacaagaa atatttgcta gctgatagtg accttaggcg acttttgaac gcgcaataat
     6061 ggtttctgac gtatgtgctt agctcattaa actccagaaa cccgcggctg agtggctcct
     6121 tcaacgttgc ggttctgtca gttccaaacg taaaacggct tgtcccgcgt catcggcggg
     6181 ggtcataacg tgactccctt aattctccgc tcatgatctt gatcccctgc gccatcagat
     6241 ccttggcggc aagaaagcca tccagtttac tttgcagggc ttcccaacct taccagaggg
     6301 cgccccagct ggcaattccg gttcgcttgc tgtccataaa accgcccagt ctagctatcg
     6361 ccatgtaagc ccactgcaag ctacctgctt tctctttgcg cttgcgtttt cccttgtcca
     6421 gatagcccag tagctgacat tcatccgggg tcagcaccgt ttctgcggac tggctttcta
     6481 cgtgttccgc ttcctttagc agcccttgcg ccctgagtgc ttgcggcagc gtgaagctga
     6541 tccgtcgaga ttaatagaaa taaattcagc ctaattcggc gttaattcag tacattaaaa
     6601 acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca caatatatcc
     6661 tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca ccactcgata
     6721 caggcagccc atcagtccgg gacggcgtca gcgggagagc cgttgtaagg cggcagactt
     6781 tgctcatgtt accgatgcta ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg
     6841 gatgatctcg cggagggtag catgttgatt gtaacgatga cagagcgttg ctgcctgtga
     6901 tcaattcggg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag
     6961 tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc
     7021 gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg
     7081 gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac
     7141 gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatggg ggaagcggtg
     7201 atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa
     7261 ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac
     7321 agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct
     7381 ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct
     7441 gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc
     7501 gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc
     7561 acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg
     7621 gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg
     7681 ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat
     7741 gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag
     7801 gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt
     7861 gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag
     7921 ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataatgt
     7981 ctagctagaa attcgttcaa gccgacgccg cttcgcggcg cggcttaact caagcgttag
     8041 atgcactaag cacataattg ctcacagcca aactatcagg tcaagtctgc ttttattatt
     8101 tttaagcgtg cataataagc cctacacaaa ttgggagata tatcatgcat gaccaaaatc
     8161 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     8221 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     8281 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     8341 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     8401 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     8461 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     8521 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     8581 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     8641 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     8701 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     8761 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     8821 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     8881 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     8941 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
     9001 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
     9061 agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg
     9121 actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     9181 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     9241 agaggttttc accgtcatca ccgaaacgcg cgaggcaggg tgccttgatg tgggcgccgg
     9301 cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta gattgcctgg ccgtaggcca
     9361 gccatttttg agcggccagc ggccgcgata ggccgacgcg aagcggcggg gcgtagggag
     9421 cgcagcgacc gaagggtagg cgctttttgc agctcttcgg ctgtgcgctg gccagacagt
     9481 tatgcacagg ccaggcgggt tttaagagtt ttaataagtt ttaaagagtt ttaggcggaa
     9541 aaatcgcctt ttttctcttt tatatcagtc acttacatgt gtgaccggtt cccaatgtac
     9601 ggctttgggt tcccaatgta cgggttccgg ttcccaatgt acggctttgg gttcccaatg
     9661 tacgtgctat ccacaggaaa gagacctttt cgaccttttt cccctgctag ggcaatttgc
     9721 cctagcatct gctccgtaca ttaggaaccg gcggatgctt cgccctcgat caggttgcgg
     9781 tagcgcatga ctaggatcgg gccagcctgc cccgcctcct ccttcaaatc gtactccggc
     9841 aggtcatttg acccgatcag cttgcgcacg gtgaaacaga acttcttgaa ctctccggcg
     9901 ctgccactgc gttcgtagat cgtcttgaac aaccatctgg cttctgcctt gcctgcggcg
     9961 cggcgtgcca ggcggtagag aaaacggccg atgccgggat cgatcaaaaa gtaatcgggg
    10021 tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct cgcggtacat ccaatcagct
    10081 agctcgatct cgatgtactc cggccgcccg gtttcgctct ttacgatctt gtagcggcta
    10141 atcaaggctt caccctcgga taccgtcacc aggcggccgt tcttggcctt cttcgtacgc
    10201 tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt ctaccaggtc gtctttctgc
    10261 tttccgccat cggctcgccg gcagaacttg agtacgtccg caacgtgtgg acggaacacg
    10321 cggccgggct tgtctccctt cccttcccgg tatcggttca tggattcggt tagatgggaa
    10381 accgccatca gtaccaggtc gtaatcccac acactggcca tgccggccgg ccctgcggaa
    10441 acctctacgt gcccgtctgg aagctcgtag cggatcacct cgccagctcg tcggtcacgc
    10501 ttcgacagac ggaaaacggc cacgtccatg atgctgcgac tatcgcgggt gcccacgtca
    10561 tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct tgggcggctt cctaatcgac
    10621 ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga ttcgatcagc ggccgcttgc
    10681 cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc gctgggcggc ctgcgcggcc
    10741 ttcaacttct ccaccaggtc atcacccagc gccgcgccga tttgtaccgg gccggatggt
    10801 ttgcgaccgc tcacgccgat tcctcgggct tgggggttcc agtgccattg cagggccggc
    10861 agacaaccca gccgcttacg cctggccaac cgcccgttcc tccacacatg gggcattcca
    10921 cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc tcctttagcc gctaaaattc
    10981 atctactcat ttattcattt gctcatttac tctggtagct gcgcgatgta ttcagatagc
    11041 agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct tcagcttggt gtgatcctcc
    11101 gccggcaact gaaagttgac ccgcttcatg gctggcgtgt ctgccaggct ggccaacgtt
    11161 gcagccttgc tgctgcgtgc gctcggacgg ccggcactta gcgtgtttgt gcttttgctc
    11221 attttctctt tacctcatta actcaaatga gttttgattt aatttcagcg gccagcgcct
    11281 ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag aacggttgtg ccggcggcgg
    11341 cagtgcctgg gtagctcacg cgctgcgtga tacgggactc aagaatgggc agctcgtacc
    11401 cggccagcgc ctcggcaacc tcaccgccga tgcgcgtgcc tttgatcgcc cgcgacacga
    11461 caaaggccgc ttgtagcctt ccatccgtga cctcaatgcg ctgcttaacc agctccacca
    11521 ggtcggcggt ggcccatatg tcgtaagggc ttggctgcac cggaatcagc acgaagtcgg
    11581 ctgccttgat cgcggacaca gccaagtccg ccgcctgggg cgctccgtcg atcactacga
    11641 agtcgcgccg gccgatggcc ttcacgtcgc ggtcaatcgt cgggcggtcg atgccgacaa
    11701 cggttagcgg ttgatcttcc cgcacggccg cccaatcgcg ggcactgccc tggggatcgg
    11761 aatcgactaa cagaacatcg gccccggcga gttgcagggc gcgggctaga tgggttgcga
    11821 tggtcgtctt gcctgacccg cctttctggt taagtacagc gataaccttc atgcgttccc
    11881 cttgcgtatt tgtttattta ctcatcgcat catatacgca gcgaccgcat gacgcaagct
    11941 gttttactca aatacacatc acctttttag acggcggcgc tcggtttctt cagcggccaa
    12001 gctggccggc caggccgcca gcttggcatc agacaaaccg gccaggattt catgcagccg
    12061 cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg gccgcgatca tctccgcctc
    12121 gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc tggtgcggtt tcatgcttgt
    12181 tcctcttggc gttcattctc ggcggccgcc agggcgtcgg cctcggtcaa tgcgtcctca
    12241 cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt cctcgctgcg ctcaagtgcg
    12301 cggtacaggg tcgagcgatg cacgccaagc agtgcagccg cctctttcac ggtgcggcct
    12361 tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg gggtgagggt agggcggggg
    12421 ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc tccgggtgcg gtcgatgatt
    12481 agggaacgct cgaactcggc aatgccggcg aacacggtca acaccatgcg gccggccggc
    12541 gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc ccgcgccggc ctcctggatg
    12601 cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca ggcggtctag cctggtcact
    12661 gtcacaacgt cgccagggcg taggtggtca agcatcctgg ccagctccgg gcggtcgcgc
    12721 ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc cggccgcgtg cagttcggcc
    12781 cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg catagcccag caggccagcg
    12841 gcggcgctct tgttcatggc gtaatgtctc cggttctagt cgcaagtatt ctactttatg
    12901 cgactaaaac acgcgacaag aaaacgccag gaaaagggca gggcggcagc ctgtcgcgta
    12961 acttaggact tgtgcgacat gtcgttttca gaagacggct gcactgaacg tcagaagccg
    13021 actgcactat agcagcggag gggttggatc aaagtacttt gatcccgagg ggaaccctgt
    13081 ggttggcatg cacatacaaa tggacgaacg gataaacct
//
